Contents of the Summer 2015 Northants News

Viscum minimum Again                                  LLoyd Gordon

Ed: In the last issue I wrote; “There are bright green, fat fruits on my Viscum. Maybe they will turn red. (They did!) Maybe they will have viable seed. (They are!) Maybe I can rush round and infect all my Euphorbia obesa. Maybe not, but it would be nice to see a project through.

I remembered I had been asked for seed many years ago and now happily posted some off to Canada, (in bubble-wrap in a CD case to reduce squashing the berries).

The recipient was Lloyd Gordon and here is Lloyd’s report...

First of all, I’d like to thank Trevor for his very generous gift of the Viscum minimum seeds.

I’ve been interested in parasitic plants for a number of years. I’ve been trying to grow various (safe species) with a small degree of success. Another generous grower sent me 2 cuttings of Euphorbia horrida (one of which died of cold damage in the mail). The cutting rooted and the Viscum minimum grew and flowered. I didn’t know which flowers were male or female and fertilization was unsuccessful. Later the Viscum started growing again but was attacked by mealy bugs and since then the inner parasite has been almost invisible.

So I was very happy to get a good number of Viscum minimum seeds from Trevor. These came at just the right time when the temperatures were slightly above zero Celsius. Since then we’ve been in a deep freeze (often -20C) and the seeds would have frozen in the mail. Anyway the seeds were plump and green and mostly still inside the plumpish berries. One even had a little radicl, (seed root), just sticking out.

Left: Two seeds of Viscum minimum germinating on Euphorbia horrida

I knew that Viscum seeds need a stable purchase on the host in order that the haustorium has the mechanical leverage to penetrate the host epidermis. So using delicate forceps, I carefully teased the seeds out of the berry along with the sticky strands of viscum. I used the viscum strands to stick the seeds to the host. I took as much of the sticky insides of the berry and used it to further secure the seeds to the host. I then discarded the skin of the berries.

The two host plants were under T5HO lights, 12 hours on/off, humidity at 25% (that’s normal for centrally heated houses in Toronto, Ontario), temperatures at 17°C lights off to 25°C lights on. I have no idea if it’s necessary but I misted the seeds with distilled water daily.

The majority of the seeds quickly produced radicles over the first 3-5 days. These over a day or 2 more made contact with the host and then formed a haustorial disc in order to penetrate the host.

 A seed of Viscum  germinating on Euphorbia ’Snowflake’

Detail of above showing the radicle forming the primary haustorium on the host cuticle.

2-3 of the seeds didn’t have enough viscum and were too loose to allow for firm haustorial contact. Using ‘5 minute’ epoxy (the kind you mix two tubes), I carefully fixed them to the host with the haustoria in close contact. I avoided getting any glue between the haustoria and the host as this would have inhibited penetration.

It’s been a few months and the seeds and haustoria seem to be doing what they are supposed to be doing. The ones on the Euphorbia polygona “Snowflake” seem very closely adherent. The two haustoria on the E. horrida are somewhat less closely adherent. Have they failed to infect the host? Or have they already done their job and the seed is ready to separate? Time will tell.

Right: Another Viscum berry germinates

It’s been very interesting watching the seeds develop and I hope I will get some Viscum growing outside the host and flowering - maybe I’ll even get some berries to share with others.

Once again, thanks to Trevor for the opportunity to grow these fascinating plants!

Lloyd

Ed: Thanks Lloyd for the article - a good swap for the seeds.

Having plenty of seeds left over I decided to sow some myself. Life is a degree more Spartan in Northants, England. In fact about 15 degrees Centigrade. The only spare Euphorbia I had  here were rooted cuts of E. mammillarias ‘Variegata’. (No, I didn’t risk the E. obesa.) I thought that should things work out the red berries of the Viscum would contrast nicely with the sickly yellow of the Euphorbia. They germinated in summer in a cold greenhouse, dipping gently in and out of frost near Christmas. When I read of Lloyd’s generous conditions I had an attack of conscience and moved them to a plus 2°C greenhouse. Whether the Viscum has actually taken up residence we will find out in due course.

Lloyd wrote recently that there is no sign of Viscum life on his hosts but the the first signs will be short stems bearing 3-part male or 4-part female flowers. Fingers crossed for both of us this autumn.

Trev

back to contents of Northants News